How To Trap MicroRNA
Researchers develop a method called miR-TRAP, which allows scientists to "freeze" miRNAs with ultra violet (UV) light giving them more time to understand the roles microRNAs play in human development and disease
Human cells are thought to produce thousands of different microRNAs (miRNAs)small pieces of genetic material that help determine which genes are turned on or off at a given time. miRNAs are an important part of normal cell function.
But, miRNAs also contribute to human diseasesome are elevated in certain tumors, for example, where they promote cell survival. To better understand how miRNAs influence health and disease, researchers first need to know which miRNAs are acting upon which genesa big challenge considering their sheer number and the fact that each single miRNA can regulate hundreds of target genes.
Enter miR-TRAP, a new easy-to-use method to directly identify miRNA targets in cells. This technique, developed by Tariq Rana, Ph.D., professor and program director at Sanford-Burnham Medical Research Institute (Sanford-Burnham), and his team, was first revealed in a paper published May 8 by the journal Angewandte Chemie International Edition.
"This method could be widely used to discover miRNA targets in any number of disease models, under physiological conditions," Rana said.
"miR-TRAP will help bridge a gap in the RNA field, allowing researchers to better understand diseases like cancer and target their genetic underpinnings to develop new diagnostics and therapeutics. This will become especially important as new high-throughput RNA sequencing technologies increase the numbers of known miRNAs and their targets."
How miR-TRAP works
miRNAs block gene expression not by attaching directly to the DNA itself, but by binding to messenger RNA (mRNA). Messenger RNA normally carries a DNA recipe out of the nucleus and into the cytoplasm, where the sequence is translated into protein.
These RNAs are bound by a group of proteins called the RNA-induced silencing complex, or RISC. This blocks production of the protein encoded by that mRNA - an action that can have far-reaching consequences in the cell.
miR-TRAP is performed in three basic steps:
1) Scientists produce highly photoreactive probes by attaching psoralen, a plant molecule that can be activated by light, to an miRNA of interest
2) Scientists perform a long-wave UV photocrosslinking reaction
3) They then pull down RNA and analyze it by RT-qPCR.
In other words, researchers zap cells with UV light, freezing the miRNA/mRNA duo in place. Then, after extracting the RNA from the cells, they take a closer look at the sequence of the bound mRNA, revealing the miRNA's target gene.
Advantages of miR-TRAP
miR-TRAP is easier and more accurate than current methods of identifying miRNA targets for three main reasons. First, miR-TRAP can directly identify miRNA targets in live cells, under normal or disease conditions.
Second, this technique can spot mRNA targets that are not only reduced by miRNAs, but also those whose translation into protein is repressed targets that aren't normally picked up by other techniques, such as qPCR or microarray analysis.
Third, miR-TRAP doesn't rely on antibodies, which can lead to nonspecific background signals and complicate data interpretation.
Putting miR-TRAP to the test, Rana and his team, including postdoctoral researcher Huricha Baigude, Ph.D., analyzed 13 predicted targets of two important microRNAs. The technique not only confirmed their known gene targets, but also revealed two novel targets.
"We're now applying these methods to identify miRNA targets in a number of disease models," Rana said. "And it's our hope that miR-TRAP will soon become common practice in many labs around the world."
This research was funded by the U.S. National Institutes of Health (NIH). The study's co-authors include Huricha Baigude, Ahsanullah, Zhonghan Li, Ying Zhou, and Tariq M. Rana.
Sanford-Burnham Medical Research Institute is dedicated to discovering the fundamental molecular causes of disease and devising the innovative therapies of tomorrow. The Institute consistently ranks among the top five organizations worldwide for its scientific impact in the fields of biology and biochemistry (defined by citations per publication) and currently ranks third in the nation in NIH funding among all laboratory-based research institutes. Sanford-Burnham is a highly innovative organization, currently ranking second nationally among all organizations in capital efficiency of generating patents, defined by the number of patents issued per grant dollars awarded, according to government statistics.
Original article: http://www.eurekalert.org/pub_releases/2012-05/smri-iat050912.php